Lab 4 Notebook Summary

Lab 4 Notebook Summary - BS111L Lab 4: Enzyme and Enzyme...

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BS111L Lab 4: Enzyme and Enzyme Reaction Rates Notebook Summary Written by Tyler Syring Lab Partner: Devan Irving
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Objectives: To test the enzyme (Sucrase) when catalyzing the hydrolysis of a disaccharide (Sucrose) into its town monosaccharide components (Glucose and Fructose). We tested the Enzyme-Disaccharide reaction in variable ranges of Temperature, pH level, and concentration of the Disaccharide (Sucrose) to find the optimal point for the reaction to proceed. Results: Temperature Range This experiment was performed using 2mL of pH 4.4 buffer, 2 mL of enzyme (Sucrase) and 2mL of the Disaccharide substrate (Sucrose) at different temperature ranges. After which the absorbance was measured at 410 nm to obtain an accurate reading of the amounts of Glucose and Fructose were produced. Also a negative control was used to zero the spectrometer, this negative control contained 2 mL of pH 4.4 buffer, 2 mL of H2O, and 2 mL of substrate (Sucrose) Curvette’s Temperature in Celsius Sample Absorbance Negative Absorbance 1 0 0.07 0 2 21 0.45 0 3 37 0.90 0 4 60 0.70 0 5 100 0.11 0
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pH Range Absorbance vs. Temperature (C) 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 20 40 60 80 100 120 Temperature Series1
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Lab 4 Notebook Summary - BS111L Lab 4: Enzyme and Enzyme...

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