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Notebook Summary1 - Results Benedicts Test Obs Result Dull...

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Results Benedicts Test: test for sugars that contain a free carbonyl group (aldehyde or ketone). Monosaccharides and some Disaccharides have these. (sucrose and polysaccharides do not) Positive results produce red color Iodine Test: reacts with linear coiled polysaccharide chains (like starch) resulting in a black or dark blue color. Branched polysaccharides (glycogen or a form of starch) have red-violet color because of incomplete helices. Negative result keeps brown color of iodine Biuret Test: Tests for presence of polypeptides. Converst dye to a violet color when positive Sudan 3 Test: high affinity for non-polar molecules like fatty acids. Benedicts Test Iodiine Test Biuret Test Sudan 3 Test Obs. Result Obs. Result Obs Result Obs. Result 1% Glucose Dull red almost Brown Positve Orange almost brown negaitve Blue Negative No change Negative 1% Sucrose Light Blue Negative Orange Almost brown Negative Blue Negative No change Negative 1% Starch Dull blue, almost green Negative Very Dark Almost Black Positive
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