Lecture_eighteen - DNA Replication, Cont`d B. C. D. E. F....

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1 A. Three possible modes of DNA replication B. Melselson Stahl Experment C. Basic Mechanics of DNA Replication D. Stages in Replication E. DNA replication Fidelity. DNA Replication, Cont’d
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2 From DNA to Protein: Genotype to Phenotype (chapter 12) I. The connection between gene to enzyme (protein) hypothesis. The gene to polypeptide II. Information Flow: The route of DNA to Phenotype. III. Transcription (rna synthesis). IV. Translation (protein synthesis).
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3 I. The connection between gene to enzyme (protein) hypothesis. A. Neurospora Crassa—fungus Chemically define its phenotype wt phenotype= sucrose, vitamins, and minerals A phenotype= sucrose, vitamins, and minerals + compound A B phenotype= sucrose, vitamins, and minerals + compound B So, they showed that they only needed to put sucrose, certain vitamins and minerals in the “growth medium”. By definition this IS the wt.
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4 B. Ariginine biosynthetic pathway and the Neurospora Crassa—fungus: Evidence of gene to protein correlation α -ketoglutarate Book’s precursor Enzyme A Ornithine Enzyme B Citrulline Enzyme C Arginine Gene A Gene B Gene C
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5 Wt phenotype: grows in the presence of media (sucrose + vitamins + minerals) with and without the presence of all other compounds.
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Lecture_eighteen - DNA Replication, Cont`d B. C. D. E. F....

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