photosynthesis lab.docx - Chiamaka Ibezimako Biology...

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Chiamaka IbezimakoBiology 111-50710/24/2018Photosynthesis AbstractAbsorption spectrumThis experiment explored the use of different wavelengths to test the absorbance of chlorophyll beta, chlorophyll alpha, xanthophyll, and Carotene. The purpose of the experiment was to investigate what wavelengths are important to photosynthetic process or photosynthesis.Action spectrumThe experiment explored the use of different lights and how efficient they are during a photosynthesis. The experiment was investigated with the use of aspirated spinach disk inside sodium bicarbonate solutions which were placed with the different colored light boxes. The experiment sought to discover which light would cause the aspirated leaves to float due to the production of oxygen which would indicate the occurrence of photosynthesis.Light intensity This experiment was conducted to examine the or measure the effectiveness of light on photosynthesis. Since light energy is required to drive photosynthesis the experiment explored different light intensity or light treatments. An apparatus was used to asses the different light intensities.
Chiamaka IbezimakoBiology 111-50710/24/2018Photosynthesis Introduction Absorption spectrumWhen photosynthesis occurs, the wavelengths of lights that are used are decided by the photosynthetic pigments. These photosynthetic pigments consist mainly of chlorophyll alpha andchlorophyll beta. Although often mistaken to be photosynthetic pigments Carotenes and xanthophylls are not really considered one. However, they are hydrocarbon chains, they use theirnon-polar hydrocarbon chains to gather pigments into the thylakoid. Xanthophylls and Carotenesare similar to chlorophyll beta and chlorophyll alpha because of their behaviors. However, chlorophyll beta and alpha are ring-like structures while xanthophylls and carotenes are not. Before conducting the experiment, it was hypothesized that chlorophyll beta and chlorophyll alpha would have the highest absorbances. The null hypothesis is that chlorophyll alpha and beta would have the lowest absorbances.Light intensity In order for photosynthesis to occur the two photosystems which are located inside a plant’s chloroplast must collaborate. Photosystems are enzymatic complexes that use light to power or allow the electron transport chain to function. This is achieved by reducing the molecules involved in the chemical reaction. Photosystem I and Photosystem II are named in the order they were discovered and not necessarily in the sequence that they occur. Photosystem II captures light energy which is used to excite electrons. The electrons are excited until they reach a high energy state they are then captured by electron acceptors. Those electrons are then moved to
Chiamaka IbezimakoBiology 111-50710/24/2018Photosynthesis photosystems II which uses them to power the electron transport chain in order for ATP to be produced. Before conducting the experiment, it was hypothesized that the tube with the light treatment 24 cm the closest to the light would have the most absorbance. The null hypothesis for

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