Orgo 1 Report 6.docx - Technique 6 Thin-Layer...

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Technique 6: Thin-Layer Chromatography: Analysis of Dyes Objective: The purpose of his lab is to understand the analytical method of TLC (Thin-Layer Chromatography). We will have known compounds to help us identify unknown compounds. They are acid-base indicators so that will help us observe how the colors are pH dependent. This is a new skill in which we are learning. Experimental Procedure: The procedure was followed as described on the Blackboard document. Additional modifications to the procedure included the following: 1. While the chromatography was taking place, we put a filter paper in the jar, and placed the plates on the inside of the paper. The reason is to make sure that the atmosphere in the beaker is saturated with solvent vapor. Saturating the atmosphere in the beaker with vapor stops the solvent from evaporating as it rises up the plate Reaction Scheme: No reaction scheme as per the Blackboard document Data and Results: TLC Plate 1: 3 Standard Dye Solutions Spot Location Dye Solution Distance traveled by solvent (mm) Distance traveled by spot(s) (mm) R f value Spot Color (Elution solvent) Spot Color (1 M HCl) Left Bromocresol green 55.0 24.0 0.440 Darker baby blue Yellow Middle Bromophenol blue 23.0 0.420 Indigo Yellow Right Bromothymol blue 30.0 0.550 Lime green Faded yellow TLC Plate 2: 2 standard dye solutions and 1 Unknown solution Spot Location Dye Solution Distance traveled by Distance traveled by R f value Spot Color (Elution Spot Color (1 M HCl)
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solvent (mm) spot(s) (mm) solvent) Left Bromocresol purple 57.1 25.0 0.440 Faded blue Dark yellow Middle Methyl red 36.0 0.630 Red and orange Deep pink Right Unknown #7 27.0/34.5 0.470/ .600 Dark baby blue/lime green Faded yellow *My unknown has 2 distances traveled by spots and R f
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