Lecture 10.ppt - Proteins make contact with bases via...

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Proteins make contact with bases via H-bonds and  van der Waals,  usually usually  within the  major   groove groove DNA does change shape upon protein binding. TATA-binding protein (TBP) is one exception. TBP binds the  minor  groove to bend the DNA thus separating the two strands.
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Denaturation/Renaturation: Strands must separate at:   1) Replication: come apart 2)  Transcription: RNA polyermase reads  one stand 3) Recombination: come apart into single  strands 4) DNA Repair: separate two strands Blue strands are complementary  to each other,  so they anneal. Red strands are complementary  to each other,  so they anneal. “anneal” Denaturation
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As DNA melts, its Abs. at 260 nm essentially doubles. T m  is dependent on the base composition. (Important for Polymerase Chain Reactions, PCRs) Fig. 5-7a
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The more  G-C  base pairs, the higher the  T m Fig. 5-7b
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Circular DNA molecules found in prokaryotes, many viruses, mitochondria, and chloroplasts.
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