Homework 2.pdf - PROBLEM 2 Sequence search problem The...

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PROBLEM # 2: Sequence/ search problem The ocean pout Macrozoarces is a saucy looking fish with a high tolerance for cold Arctic waters. In such habitats, the fish accumulates antifreeze proteins (AF) in all its tissues and body fluids. The AF was purified in microgram amounts and the following analyses were done: 1. Deconvoluted electrospray ionization mass: 6243 Da (monoisotopic) 2. N-terminus by Edman blocked 3. C-terminal CPY ladder: m 1 =128.2, m 2 =99.1, m 3 = 131.1, m 4 = 114.1, m 5 = 97.1,
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