Isolation_and_Identification_of_Biolumin.pdf - Int J Pharm...

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Int. J. Pharm. Sci. Rev. Res., 26(2), May – Jun 2014; Article No. 59, Pages: 346-351 ISSN 0976 – 044X International Journal of Pharmaceutical Sciences Review and Research Available online at © Copyright protected. Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited. 346 M. Kannahi*, S. Sivasankari PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India. *Corresponding author’s E-mail: [email protected] Accepted on: 10-05-2014; Finalized on: 31-05-2014. ABSTRACT Luminescence is the emission of light by an object. Living organisms including certain bacteria are capable of luminescence. Bacteria are the most abundant luminescent organisms in nature. Bacterial luminescence is due to the action of the enzyme called luciferase. Other interesting features of marine microorganisms are having their ability to survive at low temperatures and high salinity. In the present study, marine water samples were collected from different sites of Nagapattinam sea shores, and analyzed the physico- chemical characteristics. The effect of salinity, pH concentration on the growth and luminescence of these 10 strains was also studied. From the samples, bioluminescent bacterial strains were isolated using Sea Water Complex Agar and TCBS medium. Ten bioluminescent bacterial strains were isolated and identified based on cultural, morphological and biochemical charactertics which are belonged to the genera of Vibrio sp and Pseudomonas sp. Keywords: Marine ecology, sea water, luminescent bacteria, luciferese, oceanography, INTRODUCTION arine life is a vast resource, providing food, medicine, and raw materials. Marine organisms contribute significantly to the oxygen cycle, and are involved in the regulation of the Earth's climate. Bacteria were isolated and cultivated from all possible regions of the earth, on the basis of their habitat, diversity, ecological functions, degree of pathogenicity and biotechnological applications. 70% of the earth’s surface is covered by oceans with rich microbial diversity 1 . Bioluminescence is a form of light produced by a chemical reaction in living organisms Sluminescent bacteria are common in the ocean, especially in temperate to warmer waters 2 . Marine microorganisms have unique properties since they have to adapt to extreme marine environment conditions such as high or low temperature, alkaline or acidic water, high pressure and limited substrate in the deep sea water. These distinctive characteristics have attracted many researches to explore in depth since there is the potential of marine microorganisms used in biotechnological applications 3 . Bacterial luminescence is due to the action of the enzyme called luciferase. Excess energy is liberated in this process. The energy is dissipated as a luminescent blue-green light the bacterial luminescence reaction, which is catalyzed by luciferase,
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  • Fall '19
  • Bacteria, ISSN, J. Pharm, International Journal of Pharmaceutical Sciences Review and Research, Rev. Res.

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