Lab Report 4 FINAL.docx - CHROMATOGRAPHY Myrabelle B Lopez...

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CHROMATOGRAPHY Myrabelle B. Lopez CHEM 31.1- BC ABSTRACT Plant pigments are capable of absorbing visible light, which can be used in order to harvest energy for photochemical reactions. There are a variety of pigments present in plants such as carotenoids, flavonoids. anthocyanin and chlorophyll. For this experiment, the Aloe vera leaf was subjected to paper chromatography which is a method used by separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper. The chromatogram revealed that the only pigment extracted was chlorophyll which is essential in the photosynthetic mechanism of the plant and is responsible for its green appearance likewise, the solvent mixture of 9:1:1 (v/v) of hexane: ethanol : acetone proved to be a better mobile phase compared to the 9:1 (v/v) of ethanol and acetone.
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I. INTRODUCTION The science of organic chemistry contains a wide array of experiments that will allow us in analyzing different organic compounds which are part of our everyday lives. Chromatography is one it. It is a technique for the separation of a mixture by dissolving it in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate (Principles of chromatography, n.d.). There are many types of chromatography namely: column, planar, paper and TLC but for this experiment, paper chromatography will be utilized. According to Britannica, T. E. (2018) it is a chromatographic technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper. It involves placing a small dot or line of sample solution onto a strip of chromatography paper which is placed in a container with a shallow layer of solvent and sealed. As the solvent rises through the paper, it meets the sample mixture, which starts to travel up the paper with the solvent. This paper is made of cellulose, a polar substance, and the compounds within the mixture travel farther if they are non-polar. More polar substances bond with the cellulose paper more quickly, and therefore do not travel as far. Paper
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