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Microbial+Growth+Notes

# Microbial+Growth+Notes - Cell Growth Chapter 6 I Binary...

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Cell Growth – Chapter 6 I. Binary Fission A. DNA Replication B. Formation of Divisome 1. FtsZ 2. FtsA C. Cell Elongation 1. FtsI 2. FtsK 3. MreB D. Septum Formation E. Cell Separation II. Autolysins A. Autolysis 1

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III.Cell Wall Synthesis A. N-acetylglucosamine / N-acetylmuramic acid / pentapeptide B. Bactoprenol C. Transpeptidation IV. Growth Cycle A. Growth Phases 1. Lag 2. Exponential 3. Stationary 4. Death B. Generation ( ) Time C. Exponential Growth Time (hours) Total Number of Cells 2
1. Semi logarithmic graph 2. Practical implication of exponential growth 3. Mathematics of exponential growth N = N o 2 n = final number of cells N o = initial number of cells n = number of generations g = t/n = generation time t = time of exponential growth (hr, min) 4. Example: E. coli culture Growing exponentially for 3 hours Started with 5 cells Generation time of 20 minutes a) How many generations of E. coli are in the culture? b) What is the final cell number of the E. coli culture?

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