GMO Lab Report.docx - Kalli Rohloff Dr Bhatia LSC 348...

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Kalli Rohloff Dr. Bhatia LSC 348 November 5, 2018 Papaya is Inconclusive as Genetically Modified Abstract : GMO, genetically modified organism, is an organism that is the product of a lab process that puts genes from one species into the second organism forcibly. This is done to give the product organism the desirable traits that are going to enhance the organism. To test for the presence of GMOs we used polymerase chain reaction (PCR). We tested a papaya from Walmart in our experiment. We hypothesized that the papaya would be GMO. There were two different primers that were used to complete the PCR. One was a plant primer that could determine if the DNA from the plant was extracted successfully from the papaya. The other primer was a GM primer to determine if the extracted DNA contained GM DNA sequences. We hypothesized that the papaya would be GMO positive. In the PCR reaction there was a 455bp band in lane 4 that shows GMO positive. This is actually null due to there not being a plant primer band in lane 3. This happened since the DNA from the papaya was not extracted successfully. So this being said the papaya tested positive but it cannot be certain due to lane 3. We can say that the positive control worked since there was a plant primer band in lane 5. Materials and Methods: DNA Extraction. A non-GMO control was ground with water in a 1:5 ratio with a mortar and pestle to achieve a slurry and then 5 volumes of water was added to further dilute the mixture.
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