MicrobiologyTakehomeBiochemicalTestingProceduresNew(1).rtf

MicrobiologyTakehomeBiochemicalTestingProceduresNew(1).rtf...

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Name ____________________________________ Student ID # ______________________________ Date ____________________________________ Microbiology (Biology 2421) Laboratory Takehome Exam Biochemical Testing Procedures Value (1/2 an in-class exam grade) This exam must be enclosed in a folder and turned in no later than Wednesday, June 27, 2012. You will have to go to the lab manual and do research in order to get some of the information needed. Selective and Differential Media: 1. Define selective media: 2. Define differential media: 3. Define enrichment media: 4. List any four types of selective and differential media: 5. What makes EMB medium selective, and what does it select for? 6. What makes MacKonkey’s medium selective, and what does it select for? 7. What makes MSA medium selective, and what does it select for? 8. What does Mackonkey’s medium allow differentiation of? How? 9. What does EMB medium allow differentiation of? How? 1
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10. What does MSA medium allow differentiation of? How? 11. What type of medium is blood agar? Describe what gives the medium its properties. Describe the reactions you may observe when utilizing this medium. Carbohydrate Fermentations: 12. Define fermentation: 13. Of what practical use is the determination of an organism’s ability to ferment carbohydrates and the types of products formed? 14. Why does the laboratory manual suggest using the microorganisms it does for the carbohydrate fermentation tests? 15. Name at least two pH indicators can be used to test for the formation of acidic end products during carbohydrate fermentation tests? ? 16. What change in the physical characteristics of the medium indicate the production of acidic end products during carbohydrate fermentation? What indicates a negative reaction. 17. How is gas production occurring during carbohydrate fermentation confirmed when utilizing carbohydrate fermentation media? 18. What physical change takes place to indicate gas formation during carbohydrate fermentation? What indicates a negative reaction for gas formation? 19. List all determinations that can be made with the carbohydrate fermentation media we employed. 20. What were the five sugars employed in our carbohydrate fermentation experiments? 2
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21. If you tested an organism for its carbohydrate fermentative abilities and got the following results: arabinose positive, with acid, and gas produced; glucose negative, no acid or gas produced; lactose positive with acid but no gas produced; sucrose positive with acid but no gas produced; mannose positive with acid but no gas produced; maltose positive with acid and gas produced. What would be the observed characteristics of each of the following media employed. Assume each test medium contains a Durham tube and phenol red as the pH indicator (the same constituents we used in our lab experiments). A. Arabinose: B. Glucose: C. Lactose: D. Sucrose: E. Mannose: F. Maltose: 22. Describe the inoculation procedure for carbohydrate testing media.
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