3dView.doc - Instructions for using 3DView 1 The program...

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Instructions for using 3DView: 1 The program will accept and produce image stacks formatted as Bio-rad confocal multi-image stacks. I am in the process of developing a Mac-based image processing and analysis package which amongst other things converts tiff stacks created by NIH image into BioRad confocal stacks. The package (called ViewPoint) is currently available only from me and costs 45 $US. A demo version is available. If interested I can be contacted at ([email protected]).2 The program is not friendly at all. This is largely to make it as portable as possible. It should run on any computer regardless of the machine architecture (ie it will check for files with both types of byte-ordering). It will currently read and write only Confocal image files saved as BYTE files. It will not run correctly with WORD files. I have run the program on several Macintosh computers, but it still takes about 6 minutes to render a single view of a "virtual volume" 256X126X105 on a Centris 650. I am currently running the program on an "Alpha" made by digital, where the same view will be calculated in 3.5 to 4 SECONDS. Some rendering "methods" will be faster than others. Rendering using a "shaded surface" method and simple expansions of the image planes will be fastest. Linear interpolation and transparent pixels generally produces the most useful results, but is computationally more costly.3) Running the program. The program will simple die under several circumstances. In particular: A) The program needs to hold the entire image volume in memmory (the "real" data, not the virtual volume). If it cannot allocate enough memmory to hold the data set it will exit abruptly. B)
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