Lab Report 4 Kinetics.docx - Hanna 1 Tyler Hanna Ms Corynne Dedeo MCB 2000 Honors Laboratory 2 October 2017 Lab Report 4 Kinetics Purpose To determine

Lab Report 4 Kinetics.docx - Hanna 1 Tyler Hanna Ms Corynne...

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Hanna 1 Tyler Hanna Ms. Corynne Dedeo MCB 2000 Honors Laboratory 2 October 2017 Lab Report 4: Kinetics Purpose To determine the effect of enzymatic competitive inhibition on Vmax and Km kinetic parameters over various substrate concentrations. Data Presentation Table 3: Actual substrate concentrations Table 2: Inhibited enzyme absorbances over 70 seconds at increasing substrate concentrations Table 1: Uninhibited enzyme absorbances over 70 seconds at increasing substrate concentrations
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Hanna 2 Table 4: Uninhibited enzyme kinetic parameters and calculated Vo, Vmax, Km, and Kcat values Corrected [S] (mM) 0.0225 0.045 0.09 0.225 0.45 0.675 0.9 Slope, Δabs/sec 0.00136071 0.002388571 0.004469286 0.009011071 0.012935357 0.01391464 0.014014643 Vo, Δabs/min 0.08164286 0.143314286 0.268157143 0.540664286 0.776121429 0.83487857 0.840878571 Vo, ΔuM/min 4.44194 7.797295197 14.58961604 29.41590238 42.22641069 45.4232085 45.74965024 Vo, umoles/min 0.00066629 0.001169594 0.002188442 0.004412385 0.006333962 0.00681348 0.006862448 1/[S] (1/mM) 44.4444444 22.22222222 11.11111111 4.444444444 2.222222222 1.48148148 1.111111111 1/Vo (sec) 1500.84573 854.9973418 456.9459983 226.6347835 157.8790751 146.76785 145.720604 Non-linear regression fit Vmax =0.01017758 umol/min Km=0.32442597 mM Kcat=9.34 /min Lineweaver-Burk Vmax=0.009851246 umol/min Km=0.314569993 mM Kcat=9.04 /min Table 5: Inhibited enzyme kinetic parameters and calculated Vo, Vmax, Km, and Kcat values Corrected [S] (mM) 0.0225 0.045 0.09 0.225 0.45 0.675 0.9 Slope, Δabs/sec 0.00093429 0.001753571 0.00319 0.006095 0.009564286 0.011929286 0.013063929 Vo, Δabs/min 0.05605714 0.105214286 0.1914 0.3657 0.573857143 0.715757143 0.783835714 Vo, ΔuM/min 3.04989896 5.724389865 10.41349293 19.89662677 31.22182497 38.94217317 42.64612156 Vo, umoles/min 0.00045748 0.000858658 0.001562024 0.002984494 0.004683274 0.005841326 0.006396918 1/[S] (1/mM) 44.4444444 22.22222222 11.11111111 4.444444444 2.222222222 1.481481481 1.111111111 1/Vo (sec) 2185.86476 1164.607377 640.195054
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  • Fall '08
  • Bruno, M
  • Enzyme, Lineweaver–Burk plot, Tyler Hanna

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