BioLab Report Final.docx - Jamerial Gardner BIO 321-01 Date of Experiment Date Submitted November 6 2018 Lab Report Final Exam Cell Molecular Biology

BioLab Report Final.docx - Jamerial Gardner BIO 321-01 Date...

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Jamerial Gardner BIO 321-01 Date of Experiment: November 11, 2018 Date Submitted: November 6, 2018 Lab Report Final Exam Cell & Molecular Biology Lab Fall Semester 2018 Detection of Leukemia: Student Study & Analysis Sheets
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Objective: To perform agarose gel electrophoresis with DNA specimens in a simulation of a molecular diagnostic test for chronic myelogenous leukemia. Materials: DNA Samples (10 µ per well) Positive Control Normal Control Negative Control Patient A Patient B Size Markers 1 Cast Agarose Gel TBE Running Buffer 1X (350ml per electrophoresis chamber) Dilute WARD’S QUIKView DNA Stain, 100ml per gel 1 Gel Staining Tray 1 Set of Student Study and Analysis Sheets Electrophoresis Chamber Power Supply Micropipettes with Tips Procedure: 1. Make the buffer solution for the experiment. Add 800 ml of water with 200 ml of Tris Borate. Melt agarose gel on 1 minute and 30 seconds. 2. Taped down the glass tray sides. Be sure to not tape it down too much, only place one layer of tape on each side. After you have done this, then fold each end down.
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