buccaneer.pdf - Buccaneer The buccaneer software for...

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Kevin Cowtan, [email protected] Okinawa 2011 Buccaneer The buccaneer software for automated model building of protein structures across a broad range of resolutions. Kevin Cowtan YSBL, University of York [email protected]
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X-ray structure solution pipeline... Data collection Data processing Experimental phasing Model building Refinement Rebuilding Validation Density Modification Molecular Replacement
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Model building software: Buccaneer Model Building
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Kevin Cowtan, [email protected] Okinawa 2011 Buccaneer Statistical model building software based on the use of a reference structure to construct likelihood targets for protein features. 1.0 release in 2008 Tracing, sequencing, ncs and refinement 1.2 release (1.1.9) in CCP4 6.1.2 Faster, better sequencing 1.4 release in CCP4 6.1.13 MR support, known structure, command line 1.5 release in CCP4 6.2 Model tidying Minor sequencing improvements
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Kevin Cowtan, [email protected] Okinawa 2011 Buccaneer: Method Compare simulated map and known model to obtain likelihood target, then search for this target in the unknown map. Reference structure: Work structure: LLK
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Kevin Cowtan, [email protected] Okinawa 2011 Buccaneer: Method Compile statistics for reference map in 4Å sphere about C α => LLK target.
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