pcbp1 draft 2.docx - Josephine Flohr Hacking the Genome...

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Josephine Flohr Hacking the Genome 4/26/19 What we know of the role of PCBP1 in Biological Processes Definitions: Retrotransposons (Retrotransposed) - are genetic elements that can amplify themselves in a genome Hypomorphic - mutation that causes partial loss of gene function L-OHP - a type of chemotherapy Refractory - resistant Stemness - molecular processes including self-renewal in cells Pathogenesis - development of disease Apoptosis - controlled cell death Orthology – homologous sequences that descended from the same common ancestor Intron - non-coding aspect of DNA or RNA Isoforms - any of two or more functionally similar proteins that have a similar but not identical amino acid sequence that come from the same gene or gene family Xenograph - a tissue graft or organ transplant from a donor of a different species from the recipient. MAPK1 or ERK2 - encoded from a gene that acts as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes Oncogenesis - formation of cancer Luciferase- oxidative enzymes that produce bioluminescence We understand and have mapped to completion 28% of the human genome. The rest of us is mapped, but not fully understood. One such unknown is the PCBP1 gene and the protein it codes for, Pcbp1. Pcbp1 has implications in oncogenesis and mammalian development. This review will explore what we know about the regulation of PCBP1 and its role in biology. Poly(rC)-binding protein 1, or PCBP1, is a single stranded RNA binding protein. This protein is highly conserved across species and broadly expressed. It is found mostly in the cytoplasm and nucleus of cells. It is intron less and has three homozygous K domains, otherwise known as KH domains. The lack of introns is thought to have been created during a transposition in the gene. PCBP1 is a retro transposed derivative of another protein in the same gene family, PCBP2. The KH domains are, “Three copies of a short sequence consisting of about 70 amino acids.”(Leffers, Dejgaard, & Celis, 1995) These domains bind to RNA and have been shown to play a role in RNA function. The protein binds to the 3’ untranslated region of mRNA and regulates the translation of RNA. PCBP1 has been connected to cell apoptosis in cells containing heavily oxidized RNA(Ishii, Hayakawa, Igawa, Sekiguchi, & Sekiguchi, 2018). Along with this, PCBP1 is a part of the miRNA processing pathway that regulates miRNA biogenesis. This shows that PCBP1 plays a pivotal role in RNA regulation. The protein encoded by PCBP1 is multifunctional and has been connected to the regulation of many cancers including colon cancer. The protein has also been connected, in conjunction with PCBP2, to poliovirus RNA as well as human papilloma virus(Walter, Parsley, Ehrenfeld, & Semler, 2002). While these topics have great implications in the field of immunology as well as public
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health, I will focus this literature review on the relationship between PCPB1 and its role of regulation within the cell.
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  • Spring '14
  • WilliamBurkholder

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