Mohammed Ali 1 Lab 2 Discussion The purposes of this experiment are to identify different forms of culture media, to practice several techniques like how aseptic technique of inoculating bacteria is done, to prepare culture media and calculate its ingredients, and to know how to isolate bacteria into pure culture using streak plate method. Results, for the autoclaved sample show there was a growth of a colony in a white mucus irregular and wavy texture, and non-autoclaved tubes had no growth. There was no growth of different cultures media on the complex and synthetic medium. The MacConkey plate showed growth of E. coli with red pinkish dot of colonies and P.aeruginosa with light pink clear colony. While the soduim Azide plate showed growth of E.faecalis with whitish doted colonies and S.aureus with clear colonies. The isolated bacteria into colonies to test the pure culture technique showed no contamination and little to no single colony isolation, and the first and second quadrant were not fully covered with colonies.
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