lecture 25.3

lecture 25.3 - 25-25-1Organic Organic H BrownWilliam H...

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Unformatted text preview: 25-25-1Organic Organic ChemistryChemistryWilliam H. BrownWilliam H. BrownChristopher S. FooteChristopher S. FooteBrent L. IversonBrent L. Iverson25-25-2CarbohydratesCarbohydratesChapter 25Chapter 2525-25-3Glucose AssayGlucose Assay◆The analytical procedure most often performed in the clinical chemistry laboratory is the determination of glucose in blood, urine, or other biological fluid•this need stems from the high incidence of diabetes in the population25-25-4Glucose AssayGlucose Assay◆The glucose oxidase method is completely specific for D-glucoseOHOHHOHOCH2OHOO2 + H2OCOOHCH2OHOHHHHOOHHOHHH2O2++glucoseoxidaseD-Gluconic acidβ-∆ -Γ λυχοπ ψρανοσε(β-∆ -Γ λυχοσε2925-25-5Glucose AssayGlucose Assay•the enzyme glucose oxidase is specific for β-D-glucose•molecular oxygen, O2, used in this reaction is reduced to hydrogen peroxide H2O2•the concentration of H2O2is determined experimentally, and is proportional to the concentration of glucose in the sample...
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This note was uploaded on 03/30/2008 for the course CHEM 262 taught by Professor Forbes during the Spring '08 term at UNC.

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lecture 25.3 - 25-25-1Organic Organic H BrownWilliam H...

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