lecture 25.3 - Organic Chemistry William H Brown...

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25- 25-1 Organic Organic Chemistry Chemistry William H. Brown William H. Brown Christopher S. Foote Christopher S. Foote Brent L. Iverson Brent L. Iverson
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25- 25-2 Carbohydrates Carbohydrates Chapter 25 Chapter 25
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25- 25-3 Glucose Assay Glucose Assay The analytical procedure most often performed in the clinical chemistry laboratory is the determination of glucose in blood, urine, or other biological fluid this need stems from the high incidence of diabetes in the population
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25- 25-4 Glucose Assay Glucose Assay The glucose oxidase method is completely specific for D-glucose OH OH HO HO CH 2 OH O O 2  +  H 2 O COOH CH 2 OH OH H H HO OH H OH H H 2 O 2 + + glucose oxidase D-Gluconic  acid β - ∆ -Γ λυχοπψρανοσε (β-∆ -Γ λυχοσε29
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25- 25-5 Glucose Assay Glucose Assay the enzyme glucose oxidase is specific for β -D- glucose molecular oxygen, O 2 , used in this reaction is reduced to hydrogen peroxide H 2 O 2 the concentration of H 2 O 2 is determined
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