lecture 25.3

lecture 25.3 - 25-25-1Organic Organic...

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Unformatted text preview: 25-25-1Organic Organic ChemistryChemistryWilliam H. BrownWilliam H. BrownChristopher S. FooteChristopher S. FooteBrent L. IversonBrent L. Iverson25-25-2CarbohydratesCarbohydratesChapter 25Chapter 2525-25-3Glucose AssayGlucose AssayThe analytical procedure most often performed in the clinical chemistry laboratory is the determination of glucose in blood, urine, or other biological fluidthis need stems from the high incidence of diabetes in the population25-25-4Glucose AssayGlucose AssayThe glucose oxidase method is completely specific for D-glucoseOHOHHOHOCH2OHOO2 + H2OCOOHCH2OHOHHHHOOHHOHHH2O2++glucoseoxidaseD-Gluconic acid- - (- - 2925-25-5Glucose AssayGlucose Assaythe enzyme glucose oxidase is specific for -D-glucosemolecular oxygen, O2, used in this reaction is reduced to hydrogen peroxide H2O2the concentration of H2O2is determined experimentally, and is proportional to the concentration of glucose in the sample...
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lecture 25.3 - 25-25-1Organic Organic...

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