photosynthesis

photosynthesis - Jenny Conrad Photosynthesis: The Effect of...

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Jenny Conrad Photosynthesis: The Effect of Light Intensity on Hill Reaction Rate Abstract: In this experiment, we investigated the effect of light intensity on the rate of the Hill reaction by isolating chloroplasts, by cell fractionation and differential centrifugation. Our results showed that the absorbance at 600nm decreases as time passed, regardless of the light intensity. We then found that as the light intensity decreases, the Reaction rate decreases. Introduction: I predicted that as the light intensity increased, the absorbance and reaction rate would also increase. Material and Methods: Isolation of Spinach Chloroplasts Wash spinach leaves, discard unhealthy tissue, and remove major veins with a razor blade. Cut remaining material into 10mm square pieces. Weigh about 12g of tissue. Homozenize the tissue: Transfer the leaf pieces to a blender. Prepare a mixture of “isolation medium” and ice prepared by placing about 150ml of cold double-strength isolation medium (.8M sucrose, .06M KCl, and .04M Tricine buffer at pH 7.5) in a beaker and adding crushed
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photosynthesis - Jenny Conrad Photosynthesis: The Effect of...

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