cftp-L7.pptx - COMPLEMENT FIXATION and NEUTRALIZATION TEST Payal Bhatnagar Date Specific Learning Outcomes At the end of this lecture students should be


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Specific Learning Outcomes At the end of this lecture, students should be able to: Explain the basic principles of Complement fixation test and neutralization test. Describe the procedure of Complement fixation test, neutralization test and their uses.
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Introduction The complement fixation test (CFT) was extensively used in syphilis serology after being introduced by Wasserman in 1909. Complement is a protein (globulin) present in normal serum. Whole complement system is made up of nine components: C1 to C9 Complement proteins are heat labile and are destroyed by heating at 56°C for 20 – 30 minutes. Complement binds to Ag-Ab complex When the Ag is an RBC it causes lysis of RBC’s.
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Principle Complement takes part in many of the immunological reactions. It gets absorbed during the combination of antigens and antibody. This property of antigen–antibody complex to fix the complement is used in complement fixation test for the identification of specific antibodies. The haemolytic system containing sheep erythrocytes (RBC ) and its corresponding antibody (amboceptor ) is used as an indicator which shows the utilization or availability of the complement. If the complement is fixed then there will be no lysis of sheep erythrocytes, thus denoting a positive test . If the complement is available then there will be haemolysis which is a property of complement, denoting a negative test .
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The test requires five reagents and is carried out in two steps.
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  • Fall '15
  • MrRosdi
  • antigen-antibody complex

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