aplikasi_filogenetik_mol_topikh_23012017.pdf - Aplikasi...

Aplikasi analisis
filogenetika molekuler
Topik Hidayat
Biologi-UPI Bandung
SITH-ITB,23 Januari 2017
Seminar Analisis Filogenetika Molekuler

(Bio)systematics
Taxonomy and
Phylogeny
Biodiversity

(Bio)systematics
Phenetic Cladistic/Phylogenetic
Molecules Non-molecules
Tree reconstruction
(Bio)systematics in practice (1)
Distance Method
Character based
Method
Parsimony
Likelihood
Bayesian

Molecule tree
Non-molecule tree
Combined tree
(Total evidence)
(Bio)systematics in practice (2)

Monophyletic
group
outgroup
plesiomorphic
sinapomorphic
apomorphic
Phylogenetic
Classification
System
(Bio)systematics in practice (3)

Data:
– Morphology
– Anatomy
– Palynology
– Microscopy
– Biochemistry
– Physiology
– Ecology
– Molecular/genetics/Omics

Types of molecular data?
• DNA sources:
- Nucleus
- Chloroplast
- Mitochondria
• Methods:
- Restriction
- Sequencing
Selection of sources
and methods are
depending on the
issues that will be
addressed.

DNA sequences

Filogenetika Molekuler ?
Program komputer : PAUP & MrBayes
& Phylip
Karakter molekuler
1 60
M. longipes GGTAGAACGCAACCGGCACGACAGGATCCACT-AGGATGTTTTTCAAACGCCCACCGACT
M. altissima GGGAGAACGCAACCG-CACGACATGATC-ACT-AGGTTGTTTTTCAAACGCCCACCGATT
M. alstissima var . GGGAGGACACTTCCCGCGTGACG-GGATCACT-AGGTCGTTTCT-RAACGCCCACCGATT
M. applanata GGGAGAACGCAACC-GCACGACATGGGTCACTTAGGTTGTTTTTCAAACGCCACCCGACT
Rhus succedanea GGGAGAACGCCGCCCGCACGACAGGATCCACT-AGGTTGTGTTTCAAACGGCCACCGATT
Rhus taitensis GGTAGAACACGAACCGCACGACCAGGATCACT-AGGTTCTGTTTCAAACGGCCACCGATT
Rhus sp. GGGAGAACGCAACCGGCGCGACACAATCCACT-AGGTCGTCTTTCGAACGCCCACCGATC
** ** ** * * * *** *** *** * * * **** * ****
61 120
M. longipes GTTCGCGGTTTAAGCGTCACCGAGAACTCAGATTTGGGCCAACCGGCACGGGCGAGAGCA
M. altissima GT-CGCGGGA-AAGCGTCACCGAGAACTCACATTTGGGCCAACCCGCACGGGCGAGCGCA
M. altissima var. GT-CACGGR--AAGCGTCACCGAGAGCTCGGGTTTCGGCCAACC-GCGCGTGCGAGCGCG
M. applanata GT-CGCGGGGAAAGCGTCACCGAGAACTCATATTTGGGCCAACCCGCACGGGCGAGCGCA
Rhus succedanea GTTCGCGGATTAAGCGTCACCGAGAACTCTTATTTGGGCCAACCCGCGGGCGCAAGCACA
Rhus taitensis