Western Sydney University 2019 300802 Biodiversity Prokaryotic and Eukaryotic Microorganisms45 Practical Manual EUKARYOTIC AND PROKARYOTIC MICROORGANISMS Background reading Mason KA, Losos AB, Singer SR, Raven PH, Johnson GB 2017 Biology, 11th ed. McGraw-Hill, New York.•Chapter 28 Prokaryotes •Chapter 29 Protists •Chapter 31 Fungi At the completion of this Practical you should be able to: •Identify the differences between prokaryotic and eukaryotic microorganisms •Observe bacteria and identify the various nutritional types •Observe structural features of the protist Paramecium•Identify the major structural features of fungi 3.1 Introduction In this practical, we will observe microorganisms and the defining features that are used in their identification. All organisms can be classified into three domains of life, Eukarya, Bacteria and Archaea. All Bacteria and Archaea are microscopic, prokaryotic organisms which are generally unicellular. They play important roles in most ecosystems, many recycle nutrients, convert CO2into organic material (while simultaneously producing oxygen) and provide food for organisms in the higher trophic (feeding) levels. The domain Eukarya includes both microscopic and macroscopic organisms, which are made up of eukaryotic cells. Eukarya consists of four kingdoms, Animalia, Plantae, Fungi and Protista. As you will learn from lectures, Protista is not a true kingdom, rather a collection of eukaryotic organisms that do not fit into any of the other kingdoms. To learn more about the prokaryotic and eukaryotic microorganisms belonging to the domain Bacteria and kingdoms Fungi and Protista, you will observe them microscopically. You will learn to identify important features which are useful in identifying each group. You will also look at examples of the different nutritional types of Bacteria. You will be working with microbial cultures, you must wear safety goggles when directed during this practical class.