CBLG311: Cell Biology S2019Professor: Dr. Roberto Botelho1
MDCK cell monolayer undergoing wound closure. Image courtesy of Assaf Zaritsky and Doron Kaplan. Cells moving (migrating) to repair a wound2
Image courtesy of George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (J Cell Biol. 2009 Dec 14;187(6):831-45.)Cell Division3
Section 1: Introduction to Cell BiologySection 1: Introduction to Cell BiologyReference reading (6e) : Chapter 1: pgs: 1-15; 24-28; Chapter 2: pgs: 43-50; 51-55; 57; 60-66; Panel 2-1, 2-2, 2-3, 2-7; Chapter 3: pg. 109-119; 134-136; 143-158;1.Key concepts about cells Chemical information DNA replicationTranscription and types of RNAsTranslationCellular Energy2.Prokaryotes vs. eukaryotes: what are they and their differences3.Evolution of eukaryotic cells4.Chemical composition of cellsSmall moleculesMacromolecules (types, diversity)Macromolecule TypesShape determines function5. Protein folding and structureAmino acids and polypeptides Ligand-protein interactionskinases, phosphatase and GTPases4
WHAT IS CELL BIOLOGY?The study of the cell occurs at many levels:•Molecular composition and organization•Subcellular structure and organization•Function of the individual components of the cell•Cell differentiation and function•Communication between environment and cells•Cell replication5What?Where?How?
TO UNDERSTAND CELLS:A modern cell biologist understands and uses:•Molecular genetics•Recombinant DNA and genetic engineering•Biochemistry•Genomics and proteomics•Systems biology•Bioinformatics•Advanced microscopy and imaging6
What’s a Cell?The smallest unit of life. Inside the cell:Order and structureInside the cell:Order and structureThe non-living worldPlasma membrane:Separates the living from non-living worldsNutrients,Energy, information7
Some universal properties of cells•The __________________ delineates the cell and controls the exchange and communication with the non-living world that surrounds the cell.•The inside of the cell is ordered or disordered? ________________•Cells ________________ to form two new cells: continuation of life•This is possible because of the genetic code stored chemically as DNA•The DNA code is faithfully replicated by ___________________•The DNA encodes discreet units of of information: _________________•Genes are ______________ into discreet molecules: RNA•RNA is then ________________into proteins, which are the workhorses of the cell. Reference reading: Chapter 1, pages 1-118