Protein (2).docx - Charles1 Running Head PROTEIN CONTENT =...

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Running Head: PROTEIN CONTENTCharles1=Protein ContentMalika Charles9/24/18Dane BenedictBiology Lab 2107Georgia State University
Protein Content Charles 2AbstractThis protein concentration determination experiment utilizes the Bradford protein assay spectroscopic analytical procedure. This experiment aims to determine the concentration of three known protein solution Muscle Milk, Fairlife Milk, and whole milk. Then draw a standard curve by plotting the concentration and absorbance. Also, absorbance is the dependent variable and is plotted on the y-axis. Concentration the independent variable is plotted on the x-axis. Standards were prepared by adding 1 ml of Coomassie Brilliant Blue Dye into seven cuvettes and various measurements of Phosphate-buffered saline (PBS). The determination of the protein unknown concentration three samples of milk (Muscle Milk, Fairlife Milk, and whole milk) required 1 ml Coomassie Brilliant Blue Dye of and 20 μl of each sample. Through the use of the spectrophotometer, the absorbance’s (OD) for the known proteins were determined.Keywords:• Protein• Bradford Assay Method• Spectrophotometer
Protein Content Charles 3IntroductionThe Bradford Coomassie method was created by Marion M Bradford in 1976. This spectroscopic analytical procedure is based on the reaction amino acid composition of a solution containing protein. The Bradford Coomassie brilliant blue assay is the most common method used for quantification of protein content in a solution. The determination of protein quantities ina solution is accomplished by the measurement of light absorbance at a given wavelength (Ernst & Zor, 2010). This laboratory experiment required the use of a spectrophotometer, cuvettes, and micropipettes. A spectrophotometer is a device that utilizes a light source at a particular wavelength that is emitted through a solution of known or unknown protein concentration. Then spectrophotometer gives a numerical value which is known as the absorbance. The unit of measurement for absorbance is optical density (OD) (Gutzler & Brewer, 2018). The wavelength of light that is used in this experiment is 595nm. The solutions are whole milk, Fairlife Milk, and Muscle Milk. The manufactures protein concentration on these protein solutions are as follows: whole milk 33.3 mg/ml, Fairlife Milk 54.2 mg/ml, and Muscle Milk 60.4 mg/ml. The protein concentration in Muscle Milk is higher than whole milk, and Fairlife Milk, therefore, its absorbance will is less than the other protein solutions.

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