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Chapter 14 Practice Problems

Chapter 14 Practice Problems - BICH 411 Chapter 14 Practice...

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BICH 411 Chapter 14 Practice Problems Compiled by Dr. L Mullins Jan 2006 Biochemistry 411 Chapter 14 Key These are problems that have been given as homework, quiz and exam questions. All answers should be correct, but there is always a possibility that I miss- typed an answer. So work out to verify!! 1. List the reactions of glycolysis that: (a) are energy consuming under standard conditions. Phosphoglucoisomerase G o ’ = 2.2 kJ/mol Aldolase G o ’ = 22.8 kJ/mol Triose phosphate isomerase G o ’ = 7.9 kJ/mol GAPDH G o ’ = 6.3 kJ/mol Phosphoglycerate mutase G o ’ = 4.4 kJ/mol (b) are energy yielding under standard conditions. Hexokinase G o ’ = -20.9 kJ/mol PFK-1 G o ’ = -17.2 kJ/mol Phosphoglycerate kinase G o ’ = -14.2 kJ/mol Enolase G o ’ = -3.2 kJ/mol Pyruvate kinase G o ’ = -23.0 kJ/mol (c) consume ATP. Hexokinase G o ’ = -20.9 kJ/mol PFK-1 G o ’ = -17.2 kJ/mol (d) produce ATP. Pyruvate kinase G o ’ = -23.0 kJ/mol Phosphoglycerate kinase G o ’ = -14.2 kJ/mol 2. Examine the five glycolytic intermediates: 2PG 1,3-BPG pyruvate GAP 3PG A. name each intermediate B. write the order in which they appear in glycolysis - D,B,E,A,C C. Which intermediate can react in substrate-level phosphorylation - B D List the glycolytic enzyme for which each intermediate is a substrate Enolase converts 2PG to PEP, PGK converts 1,3-BPG to 3PG, LDH converts pyruvate to lactate or pyruvate decarboxylase converts pyruvate to acetaldehyde, GAPDH converts GAP to 1,3-BPG, and PGM converts 3PG to 2PG 3. How many kinases reactions are found in glycolysis? What are the names of the enzymes?
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