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Tanisha SharmaBSCI 202 LabReport:BreathingandHeartRateiWorxAssignmentIntroduction:In this experiment, we will first determine the heart rate and the respiratory sinus arrhythmia (RSA) of a subject breathing at rest and then determine the effect of apnea on the subjects heart rate. RSA is the naturally occurring cyclic change in heart rate thatoccurs during a breathing cycle (at rest). As you breathe normally, heart rate increases during inhalation and decreases during exhalation (Hayano). We used a spirometer for both parts of the experiment. A spirometer is a device that monitors breathing from a subject by recording the amount of air you breathe in and out and the speed of your breath (Restrepo).For the first part of the experiment, the purpose was to determine the effect of breathing (at rest) on a subject’s heart rate and the change in their heart rate during RSA. First, we had an assistant hold the spirometer vertically in front of the subject so the outlets are pointed up in order to obtain accurate measurements. We then had the subject sit quietly and adjust the flow head of the spirometer to be at mouth level. We then begin to record. After waiting about five seconds for the volume channel to zero out, the subject put the flow head in their mouth and began breathing normally. We recorded the breathing for five breaths (~forty-five seconds) and then stopped. Lastly, we place one cursor on the trough before the first breath cycle and the second cursor to
the trough before the second breath cycle and use the functions iWorx program to find the Tidal Volume (TV), Minimum HR, Maximum HR, mean HR, and RSA in between these troughs. We repeat these measurements on two other breath cycles. We recorded all the measurements for each of the three cycles. For the second part of the experiment, the purpose was to measure the effect of apnea (when you stop breathing), after a maximum inhalation, on the subject’s heart rate. Just like in the first part, an assistant holds the spirometer vertically in front of the subject at mouth level. After we press record we also wait five seconds for the volume channel to zero. Then the subject takes two to three normal breaths, after that the