Lab3_PostLab

Lab3_PostLab - separation, (2) worst separation, and (3)...

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Chem 2545 Fall 2007 Post Lab 3 Report I. Title of lab II. State the objective(s) of the lab III. Give a summary of your observations IV. Postlab Questions/Discussion of Results Part I-Spinach Extraction 1. Draw all of the TLC plates that you developed. Label each plate with the appropriate solvent system. Include R f values for each plate. 2. (a) Which solvent system gave the best results? Explain. (b) What was wrong with the other solvent systems? Explain. 3. Describe how the polarity of the solvent system affected the number and color of spots that were observed. 4. Why was hexane used as a spotting solvent for the extracted pigment? Part II-Separation of four organic compounds 5. Draw three TLC plates. Draw the plate which gave the (1) best
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Unformatted text preview: separation, (2) worst separation, and (3) any other plate of your choice. State the solvent system for each plate. 6. (a) List all the solvent systems that you tried. (b) Which solvent system gave the best overall separation? Explain. (c) State your original solvent system and explain your rational for the other Chem 2545 Fall 2007 solvent systems you tried. Explain what observations led you to your best solvent system. 7. Use a drawing to illustrate how triphenylmethanol and benzophenone interact with silica gel. V. Calculations 8. Calculate the R f values of the plate that gave the best separation in Part II, Question 5. Show all your calculations....
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This note was uploaded on 03/31/2008 for the course ? ? taught by Professor ? during the Fall '05 term at Indian Hills CC.

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Lab3_PostLab - separation, (2) worst separation, and (3)...

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