Micro Lab 4

Micro Lab 4 - microbiology lab In addition these techniques...

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Flora Vo 24 September 2007 TA: Adrianna 2-7 Lab 4: Pure Culture Isolation, Conjugation, and Bacteriophage Titration 1. What are we doing today? a. Observing the culture transfer and agar streak plates from the previous lab. b. Making a pure culture isolation by streaking another agar plate with a single colony grown from last week’s lab c. Conjugating two strains of Escherichia coli where strain I was a chromosomal gene for resistance of streptomycin. Strain II has a plasmid for resistance to ampicillin. d. Learning how to do a bacteriophage titration and then, plating it to later observe the formation of plagues. 2. Why is this important? a. Correctly transferring bacteria cultures and correctly isolating bacterial colonies on agar streak plates are essential techniques one needs to master for
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Unformatted text preview: microbiology lab. In addition, these techniques are also used to research unknown samples of bacteria in labs and hospitals. b. Conjugating E. coli will enable us to visualize horizontal transfer, which can quickly display selection pressure for a specific trait, such as antibiotic resistance. Also, we can see the dangers of overtaking antibiotics because it kills the weak bacteria while selecting a strain of resistant bacteria, a problem in our current time. c. This specific bacteriophage titration will allow use to crudely calculate the amount of phage in the undiluted sample. In addition, the plaques will enable us to visualize the how destructive viruses can be in good environmental conditions....
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