exp07 - Standard Curves and Volumetric Glassware Laboratory...

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Standard Curves and Volumetric Glassware Laboratory Experiment #7 xxxx Section x 11/9/06
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Purpose: The purpose of this lab was to take solutions of known concentrations and determine their absorbance. By creating a standard curve with these values, the concentrations of two unknown solutions could be determined after finding each of their absorbance. Experimental Methods: In order to find the concentrations of the red dye in the two household substances, there had to be something that they could be compared to. This is where a Standard curve came in handy. By taking solutions of known concentrations and finding their absorbance, a linear regression could be fit that would describe the concentration of any solution based on its level of absorbance. A water-blank and five other samples of varying concentrations were used to create the standard curve—absorbance versus concentration. For details about which concentrations were needed and the wavelength at which the absorbance was tested, refer to the lab instructions. 100 ml of each of the five concentrations that was listed in the instructions was made using a 100-mg/L stock solution of Red #40. Data and Observations: The table below was created so that the absorbance, concentration, and sample number could be seen.
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