Section 6 - BLG311 F2019 Vesicle traffic_adjusted.pptx -...

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INTRACELLULAR VESICULAR TRAFFIC CHAPTER 13 1
Healthy cells Vesicle traffic disrupted
LECTURE SECTION: INTRACELL. VESICULAR TRAFFIC 1. Basic steps of vesicular transport Membrane fission Coats (e.g. Clathrin-coated pit assembly) Regulation of coat assembly (Phosphoinositides Coat GTPases) Targeting vesicular transport (Rab GTPases and cycle) Membrane fusion (v-SNAREs and t-SNAREs) 2. Vesicular traffic between ER and Golgi ER exit: COPII and Sar1 GTPase Retrograde traffic from Golgi to ER (COPI, Retrieval Sequences) Golgi structure and function Polarized nature of Golgi cisternae Biosynthetic processing Intra-Golgi trafficking 3. Vesicular traffic between Golgi and lysosomes Function and structure of lysosomes Degradation pathways Lysosome maturation Targeting between Golgi to lysosomes M6P receptor and its signals 4. Exocytosis Constitutive vs. regulated exocytosis, and their various functions Secretory vesicle biogenesis Global vs. localized exocytosis Balance between exocytosis and endocytosis 5. Endocytosis Phagocytosis: Functions, process, regulation and maturation Pinocytosis Fluid-phase uptake Receptor-mediated endocytosis (LDL) Trafficking and sorting early endosomes 3
4 A simple “road map” for protein transport within the eukaryotic cell
5 Vesicular transport Randy Shekman, James Rothman, Thomas C. Sudhof (Nobel Prize 2013 for Physiology or Medicine)
6 Topology of the cell Topology : continuity and ability to exchange without “crossing” the membrane
7 Functions of vesicular transport 1. Biosynthetic pathway : delivery of newly synthesized proteins and membrane from ER to most other organelles: Golgi, plasma membrane, endosomes, lysosomes 2. Exocytosis pathway : delivery of newly synthesized or stored proteins into the extracellular space: Neurotransmitters Proteolytic enzymes to kill bacteria Release of signaling peptides like growth factors and insulin 3. Endocytosis pathway: uptake of external and plasma membrane-bound proteins and ligands Vitamins and nutrients Cholesterol (LDL) Growth factors and receptors (control of cell growth) Changing the properties of the cell surface
8 Vesicle traffic at the plasma membrane: Exocytosis and endocytosis
9 Roadmap of the endocytic, biosynthetic and recycling vesicular pathways
10 Four major types of routes for membrane vesicles within cells (1) Biosynthetic pathway (2) Endocytosis – degradation pathway (3) Endocytosis – recycling pathway (4) Membrane retrieval pathway
11 There is constant and rapid membrane flow between organelles, i.e., organelles are effectively continuous

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