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DNArecomb08-1 - DNA RECOMBINATION Today's outline Left Over...

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DNA RECOMBINATION Today’s outline Left Over: telomeres NHEJ-review VDJ Recombination Proving VDJ Recombination Occurs Homologous Recombination Holliday Junction RecA Supporting evidence Using Homologous Recombination to Construct “Knock-Out” Mice Transposons
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Telomerase: Replication at DNA Ends (telomeres)
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λ Phage Uses Recombination
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Specific att sites are required Integrase is required : Topoisomerase I-like mechanism:
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Using Recombination to Clone DNA
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VDJ Recombination : How do humans fend off the nefarious world of microbial invaders? I n the 1970’s, Susumu Tonegawa and others wanted to know how B cells could produce so many different I gG molecules. Was each I gG encoded by a separate gene in the genome OR did diversity come about from a different mechanism? V = variable region C = constant region H = heavy chain L = light chain The V L and V H regions encode the part of the IgG molecule that binds the antigen
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We’ll focus on the H chain, Tonegawa’s work was on the Kappa chain.
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