DNArecomb08-1 - DNA RECOMBINATION Todays outline Left Over:...

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Unformatted text preview: DNA RECOMBINATION Todays outline Left Over: telomeres NHEJ-review VDJ Recombination Proving VDJ Recombination Occurs Homologous Recombination Holliday Junction RecA Supporting evidence Using Homologous Recombination to Construct Knock-Out Mice Transposons Telomerase: Replication at DNA Ends (telomeres) Phage Uses Recombination Specific att sites are required Integrase is required : Topoisomerase I-like mechanism: Using Recombination to Clone DNA VDJ Recombination : How do humans fend off the nefarious world of microbial invaders? I n the 1970s, Susumu Tonegawa and other s wanted to know how B cells could pr oduce so many differ ent I gG molecules. Was each I gG encoded by a separ ate gene in the genome OR did diver sity come about fr om a differ ent mechanism? V = variable region C = constant region H = heavy chain L = light chain The V L and V H regions encode the part of the IgG molecule that binds the antigen Well focus on the H chain, Tonegawas work was on the Kappa chain....
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This note was uploaded on 03/31/2008 for the course BCHM 4115 taught by Professor Emgregory during the Spring '08 term at Virginia Tech.

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DNArecomb08-1 - DNA RECOMBINATION Todays outline Left Over:...

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