Geneexp1_08 - Gene Expression 1 1. Transcription: How does...

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Gene Expression 1 1. Transcription: How does insulin, cAMP, etc. regulate PEPCK txn? 1. Methods: The Northern blot 2. A Prokaryotic Model to Understand Txn 5’ to 3’ considerations DNA-dependent RNA polymerases Promoters
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Transcription: Gene Expression, RNA Expression Our focus will be on integrating regulation at the RNA txn level to other levels of control (DNA vs. RNA vs. Protein); PEPCK as an example; prokarytic txn to understand the basics DNA structure/properties Manipulation of DNA Replication Repair Recombination The process of transcribing RNA
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PEPCK Regulated primarily at level of RNA  transcriptions.  Changes in txn =  changes in PEPCK enzyme = changes in  PEPCK activity = control of  gluconeogenesis Fed state: Insulin is high; PEPCK txn is  decreased.  Fasted state: Glucagon and cAMP are  increased; PEPCK txn is stimulated (a) Glucagon  (b) cAMP (c) Insulin (d) Glucocorticoids (cortisol, dexamethasone) Key endocrine regulators of  metabolism
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To measure steady-state mRNA levels use a Northern or RNA Blot Steady-state levels are a function of synthesis and RNA half-life How is RNA Expression Measured?
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Geneexp1_08 - Gene Expression 1 1. Transcription: How does...

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