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RecombinantDNA4_08 - Recombinant DNA 4 Today's Outline DNA...

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Recombinant DNA 4 Today’s Outline DNA sequencing Libraries: Genomic cDNA Reverse Transcriptase Screening Libraries Transgenic Organisms
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DNA Sequencing 198 0 Professor Paul Berg of Stanford University in California received one half of the $212 ,000 prize in chemistry. The other half w ent j ointly to Professor Walter G ilbert of Harvard and P rofessor Frederick Sanger of Cambridge University. Gilbert and Sanger were cited b y the award ing bod y "for t heir contributions concerning the determ ination of ba se sequences in nucleic acid s." The Rxn: DNA template ss primer Nucleotides DNA polymerase (requires ds DNA portion) From Ch 11
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Making Libraries: Genomic DNA
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Alternative vector systems for Libraries Approximate maximum length of DNA that can be cloned into vectors Vector type Cloned DNA (kb) Plasmid 20 Lambda phage 25 Cosmid 45 BAC (bacterial artificial chromosome) 300 YAC (yeast artificial chromosome 1000
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Cosmid Libraries
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cDNA Libraries
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197 5 The prize was awarded jointly to:
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