RNAilecture08 - History of RNAi: Worms and Plants + 35S...

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The color purple: The phenomenon of "co-suppression" was first described in petunias by Richard Jorgensen and Carolyn Napoli. 1. Plants - a transgene for chalone synthase (CS), a gene involved in pigment production, was inserted behind a strong promoter, to attempt to obtain a deeper hue of the flower. Instead the flowers grew up white or variegated. This was called "co-supression", or "quelling" in Neurospora. History of RNAi: Worms and Plants + 35S CaMVp:CS
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History of RNAi: Worms QuickTime ÷ and a TIFF (LZW) decompressor are needed to see this picture. QuickTime÷ and a TIFF (LZW) decompressor are needed to see this picture. QuickTime÷ and a TIFF (LZW) decompressor are needed to see this picture.
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What happened to the CS and lin4 transcripts???
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Animals: target mRNA degradation Plants: blocks translation
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The Extra CS transcripts “Co-Suppressed” endogenous CS mRNAs Almost perfect complementarity Less perfect complementarity
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RNAilecture08 - History of RNAi: Worms and Plants + 35S...

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