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SB0011Practical Biology ILAB REPORTEXPERIMENT NUMBER: OneTITLE:MICROSCOPY:INTRODUCTIONTOMICROSCOPYNAMEMATRIXNO.SIGNATUREIntroductionSmall and tiny organisms cannot be seen with naked eyes. They require aspecial instrument to look and observe them as they are very tiny. With every yearthat passes, the world’s technology have become more advance. As the world’stechnology develop, scientists have successfully invented useful instruments inscience and technology such as microscope.Assessment:ItemsMarks %Format____ /1Methods____ /2Resultsand data____ /2Discussion____ /3Conclusion____ /1References____ /1Total____/ 10DATE OF EXPERIMENT: 10 JULY 2019DATEOFREPORTSUBMISSION: 16 JULY 2019LAB DEMONSTRATOR: MISS RAMZIAH RAZANAH BINTI JUMATTUTORIAL GROUP: TUTORIAL 11
Microscope is an important tool that enable scientists to study specimen thatcannot be seen with our naked eyes. One of the microscopes invented was acompound light microscope. A compound light microscope uses two or more lensesto produce a magnified image of an object, known as a specimen that is placed ona slide at the base (Woodford, 2018). The image will formed when light passesthrough the specimens slide and then passes through two set of lenses which arethe objective lens and eyepiece lens.Microscope has 3 important parameters which are the magnification,resolution, and contrast. Magnification gives a large image of the specimen.Resolution of a microscope measures the clarity of the image or the ability of themicroscope to separate two closely placed objects as separate images (Chia,R.S,B.2018). In order to be seen through a microscope, the specimen should have somedegree of contrast with the surrounding medium. The contrast arises because lesslight is transmitted through the cell than through the medium. This is becausesome light is absorbed by the cell and some is refracted out of the optical path ofthe microscope by the difference in the refractive index between the specimen andthe surrounding medium (Bisen & Arjana, 2013)Objectives1.To identify the name and the function of the basic parts of the compoundlight microscope.2.To learn how to focus an object with the compound microscope.3.To measure the diameter of the field of view of each magnification power ofthe objective lens 4x, 10x, and 40xMaterialsNikon E100 compound light microscopeMicroscope slidesLetter ‘e’ from newspaper/magazineMammal spinal cord cellRulerMethods1.Identification of parts
1. A compound microscope was obtain from a cabinet and place securely on a flat table. The basic part of the microscope was identified and labeled in figure 1 2.Focusing the microscopeLowest power1.The nose piece was turned so that the lowest power lens is in straight alignment over the stage.

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