LS3%20Midterm%20IIans_3_09W - LS3 Midterm II (130 pts) Feb...

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1 LS3 Midterm II (130 pts) Feb 26, 2009 Instr. P. O'Lague NAME_________________________________ (Last) (First) I.D.# and TA____________________________ Instructions: 1. This exam has 10 pages. Be sure you have all pages. 2. WRITE YOUR NAME ON EVERY PAGE (except data sheets). 3. For TRUE/FALSE (2pts) and MULTIPLE CHOICE (3pts), mark your answers clearly. 4. For the short essay questions write answers legibly only in the spaces provided. NO CREDIT GIVEN FOR WRITING OUTSIDE THE LINES! 1. In eukaryotic transcription, ribosomal genes are transcribed by: A) Pol I and Pol II B) Pol I and Pol III C) Pol II and Pol III D) all of the above Ans: B 2. Bacterial terminating factors recognizes the following codons: A) UAG, AAU, UGA B) UGU, UAG, UUU C) UAG, UAA, UGA D) UUC, UGG, UGA Ans: C 3. Lariat structures arise from: A) branch point G-OH attacking the 3’ end of exon 1 B) branch point A-OH attacking the 3’ end of exon 1 C) branch point A-OH attacking the 5’ end of exon 2 D) branch point A-OH attacking the 3’ end of exon 2 Ans: B
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2 NAME_________________________________ 4.Which of the following events does not occur during DNA replication in E.coli ? A) Ligation of Okazaki fragments by DNA polymerase III B) Discontinuous synthesis on the lagging strand. C) Synthesis of short RNA primers. D) Attachment of SSB proteins to DNA strands. E) Unwinding of the double helix by DnaB. Ans: A 4. In eukaryotic transcription which events are necessary for promoter clearance to occur: A) binding of TBP to TATA box DNA B) TFIIF binding to Pol II. C) TFIIH phosphorylation of the CTD of Pol II D) Bending of promoter DNA by TFIID. E) All of the above Ans: E 6. Operator constitutive mutants of the lac operon would A) express the lac repressor continuously B) block the binding of RNA polymerase to the promoter C) express β -galactosidase always D) prevent the inducer from binding to the repressor Ans: C 7. An E coli cell that has the genotype I + O + Z + / I - O C Z - would A) induce non-functional β - gal upon the addition of lactose B) continuously synthesize functional β -gal C) be unable to synthesize non-functional β -gal D) repress functional β -gal synthesis in the absence of lactose Ans: D 8. The Kozak sequence is: A) ribosomal sequence used to position mRNA on the small ribosomal subunit. B)
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This note was uploaded on 05/31/2009 for the course LIFESCI LS 3 taught by Professor Paulolague during the Winter '09 term at UCLA.

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LS3%20Midterm%20IIans_3_09W - LS3 Midterm II (130 pts) Feb...

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