LS3lect11_Eucayotic%20_trans09W

LS3lect11_Eucayotic%20_trans09W - Eucaryotic Translation As...

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Eucaryotic Translation As in prokaryotic translation, protein translation in eucaryotes consists of three phases. 1. Initiation 2. Elongation 3. Termination
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* eIF4F is a complex of a cap binding protein, eIF4A and eIF4E *
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Initiation of Eucaryotic Translation 1. Of the three phases initiation differs most from that in prokaryotes. At least 9 (instead of 3) initiation factors are involved. 2. Ribosomes are larger(80S) and more complex. 3. Initiating amino acid is methionine not formyl-met. 4. mRNA’s have no Shine-Dalgarno and are monocistronic.
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Ribosome (80S) Composition Small subunit Large subunit (40S) (60S) RNA 18S 28S, 5.8S, 5S Protein’s 33 50
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General Ribosomal Binding Domains 1. 40S binds mRNA and contains the amino acyl-tRNA site (A site) and the peptidyl-tRNA site (P site). 2. 60S possesses the pepitdyltransferase domain and a GTPase domain needed for translocation.
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Characteristics of Eucaryotic mRNA 1. mRNA has two important features: an open reading frame ( a sequences of translatable
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LS3lect11_Eucayotic%20_trans09W - Eucaryotic Translation As...

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