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PS2B - BICD100 Genetics W08 Problem Set 2B Chisholm Problem...

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Problem Set 2B Tetrad Analysis 1. From a cross of a trp1 x α trp + you sporulate and dissect 300 tetrads. You get two classes as below. Determine the genetic map. I II a trp1 a trp+ a trp1 a trp+ α trp+ α trp1 α trp+ α trp1 157 153 2. The yeast cross a leu2 x α leu+ produces the following tetrads. Determine the genetic map. I II a leu2 a leu2 a leu2 a leu+ α leu+ α leu2 α leu+ α leu+ 127 15 3. You cross two haploid yeast strains, the triple mutant his2 leu1 cdc5 x his+ leu+ trp+ (i.e. wild type) to obtain a triply heterozygous diploid which you then sporulate to obtain tetrads. The his2 gene is tightly linked to the centromere of chromosome VII. The leu1 and cdc5 genes are both located on the same arm of chromosome III, with leu1 closer to the centromere than cdc5 . For each of the following types of tetrads, diagram the meiosis at the 4-chromatid stage. The diagram should show the location of crossover(s) if any, relevant centromeres, and
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