Photosynthesis Lab

Photosynthesis Lab - Photosynthesis SEC TA NAME DATE 410...

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Photosynthesis SEC 410 TA Janairo NAME Spenser Jacobson DATE October 1, 2007 Pledge: This work is solely of my creation. No unauthorized assistance was received in its completion
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Photosynthesis is a process in which photosynthetic organisms trap solar energy and convert it into usable energy. Such organisms include some bacteria, some protists and most obviously all plants. In order for this reaction to occur, water, carbon dioxide, and light must be present and the organism must contain chloroplasts. The products of photosynthesis are carbohydrates and oxygen. In other words, the photosynthesis equation is expressed as: H 2 O + CO 2 + light (CH 2 O) + O 2 There are two different sub-reactions of photosynthesis. One occurs in the thylakoid membrane of the chloroplasts. This first reaction is called the Light Reaction, or commonly called the Hill Reaction. During this time, light energy and water form ATP and oxygen. In addition, NADP + is being reduced to form NADPH. NADPH is necessary as a resource for the impending light dependent reactions. However, in this lab, DPIP is used as a substitute for NADP + . DPIP can be used because its color transforms from blue to colorless when it is incubated with chloroplasts that are in the process of photosynthesis. The color change is due to the reduction of DPIP, meaning it accepted electrons. The second “dark” reaction takes place in the stroma, the fluid within the chloroplasts which surrounds the thylakoid. ATP, NADPH, and carbon dioxide produce sugar, ATP, and NADP + . Plants, for the most part, appear green; we know this to be true because of the pigment chlorophyll found in chloroplasts of a plant cell. However, this knowledge goes further and involves the visible light spectrum (ROYGBIV). The explanation of why plants look green is that every frequency of the visible light, except for the one associated with green, gets absorbed. The independent variable for this experiment was the different colored light used, and the
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This note was uploaded on 03/31/2008 for the course BIOL 101L taught by Professor Stengaga during the Fall '08 term at UNC.

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Photosynthesis Lab - Photosynthesis SEC TA NAME DATE 410...

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