Part 1:
1.
Turn on the spectrophotometer, set the wavelength to 540 nm., and allow it to warm
up for fifteen minutes.
2.
Set up 4 cuvettes with the appropriate contents, make sure to add the enzyme last
(do not label in a place that will alter the results from where the spectrometer’s
emission light goes):
a.
Cuvette 1: 2 mL. catechol solution, 3 mL. distilled water.
b.
Cuvette 2: 1 mL. enzyme solution, 4 mL. distilled water.
c.
Cuvette 3: 2 mL. catechol solution, 1 mL. enzyme solution, 2 mL. distilled
solution.
d.
Cuvette 4: 5 mL. distilled water.
3.
Cover tubes with parafilm, and invert them all.
4.
Use cuvette 4 to calibrate spectrophotometer and then get absorbency levels for all
cuvettes.
5.
After getting readings, place cuvettes into a 37 degree Celsius water bath.
6.
Shake cuvettes at five minute mark.
7.
After ten minutes, remove cuvettes, wipe exteriors with Kimwipes and place cuvette
4 into spectrophotometer.
8.
Re-calibrate the machine and then get absorbency readings for all cuvettes.
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- Fall '08
- Stengaga
- Biology, Enzymes, Fahrenheit, cuvette, Chelation, cuvettes, Chelating agents
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