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Lab 1 Microsope Usage 2008

Lab 1 Microsope Usage 2008 - Biology 1111 Lab 1 part a...

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Biology 1111 Lab 1 part a: MICROSCOPY A. Introduction The first topic of this lab is microscopy and basic observations . You will learn the parts of the compound light microscope, how it functions and then use the microscope to do several exercises with prepared and live specimens. The compound scope and the stereo dissection scope will be used again later labs. You will need these skills in upper level biology courses as well. If you have used a microscope before, some of this will be a review. Help your lab partners if they are new to the microscope. Group work is an important component of 1111 lab. All labs in Biology 1111 (as well as other Biology lab courses) require advanced preparation. To aid you in your preparation you should review the lab manual and prepare what is called a protocol for the lab; basically an outline of the steps of the exercise that you can easily refer to at the lab bench. If a write up or formal lab report is required for a lab, the protocol becomes the methods section. More information on writing a protocol is available on Blackboard Some general rules for the use and care of this instrument are as follows: 1. Always carry a microscope with both hands and in an upright position. One hand grasps the arm and the other supports the base. 2. Use only lens paper for cleaning the lenses. Lens paper is on your bench. Soak the lens paper with alcohol and clean the ocular and objective lenses, then dry. Most problems with observing an image clearly relate to dirty lenses – clean the lenses at the start. 3. When using the microscope, always start on the lowest magnification. Raise the stage to its highest position looking from the side of the scope NOT through the ocular lens or eyepiece. When focusing, turn the focusing (fine or coarse) so the stage lowers. This prevents the slide from hitting and damaging the objective lens. 4. Before the scope is put away , swing the lowest power objective into position, raise the stage to its highest position . Center the slide mover . Check to see that there are no slides left on the stage. Reduce illumination intensity to the minimum and turn off the power switch. Make sure the scope is clean, particularly if the oil lens was used. 5. You will be assigned the same scope each laboratory and will have to sign it out. If anything is wrong with your scope, report it to your instructor immediately. If there is a problem and you do not report the problem immediately, you may be held responsible for any damage. Return the scope to its numbered bin with the stage lowered, slide holder centered, lower power objective in place, the cord wrapped around base neatly and the scope dry and clean. If it is not in this condition when you remove it from its bin, let your lab instructor know immediately so you don’t have to take the rap.
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