Lab 1 Bacteria Lab set-up protocol 2008

Lab 1 Bacteria Lab set-up protocol 2008 - Lab 1 Partial...

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Lab 1 Partial protocol; Bacteria in the Environment (use in place of lab manual) Introduction This Bacteriology laboratory covers two labs. In the first lab, you will set up the exercise and in the second lab, you will observe the results of your efforts. The first exercise (ex. 3, p. 14 in Symbiosis) studies different environments, testing for the presence of bacteria and fungi in these environments to study diversity in organisms. . Each bench will investigate five different environments and visually determine the general types of organisms found in the different environments. An optional environment for testing may be assigned. You will learn about sterile technique and use this to inoculate the agar plates. The second exercise (ex. 4, p. 21 in Symbiosis) investigates control of microorganisms through the use of antibiotics, antiseptics and disinfectants. The set up of the plates require a technique to spread the bacteria on to the agar to get an even distribution called a “lawn”. The following is a protocol, something you will have to do for other labs, but more details as you may have to use this as your primary source of information if you don’t have a lab manual. You should be able to perform any lab directly from your protocol as if you did not have a lab manual. Materials Sterile agar plates (NA) Environmental samples Wax pencil raw chicken (food supply) Sterile cotton tipped swabs soil (natural environment) Inoculating loop stream or pond water (potable?) Alcohol or Bunsen burner hands (yours) Sterile water Air (that you breathe) Investigation Before you begin, read the instructions to the exercise for the manual or handout. Use Campbell for background information about bacteria. Consider the following questions: Where in the environment would bacteria be most common? How about other microorganisms such as fungi? Should any of these environments be free of organisms such as bacteria or fungi? What are you basing our answers to the above questions on? You have not done your investigations yet. Using what you know from previous observations or experience, you are making a hypothesis. In the discipline of science, for a hypothesis to be of any use, it has to be testable, or the predictions you make based on the hypothesis have to be testable. Make a formal hypothesis (usually in an if/then statement format) and predict the results of your
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Lab 1 Bacteria Lab set-up protocol 2008 - Lab 1 Partial...

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