HW key - 1 BIOL105ProblemSet#7 KEY Name DS TA Textbookproblems ,problems3,4,8,11,12,15a,b,16,17,18,19,21,26 ,problemsE5,E6,E7 Idon',

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BIOL105 - Problem Set #7 KEY Name _______________________ DS # _______________________ TA ______________________ Textbook problems: Chapter 11 Solved Problem I, problems 3, 4, 8, 11, 12, 15a,b, 16, 17, 18, 19, 21, 26 Reference E solved problem I, problems E-5, E-6, E-7. I don't have a good stockpile of extra RFLP questions, so instead am giving you quite a few (some quite challenging) from the book and "for credit", to give you lots of practice. "For credit" problems are due Tuesday, May 27 by 8:00 am. You can turn them in to the drop box outside Sinsheimer or give them to me BEFORE class on Tues. 1) Spinobulbar muscular atrophy is a dominant disease due to expansion of a CAG repeat in the gene encoding androgen receptor (AR). Normal individuals have a small number of CAG repeats, e.g. 11 repeats in their AR gene. Affected individuals have an expanded number of repeats, e.g. 64 repeats in one allele of the AR gene. You want to use PCR to amplify the CAG- containing region of AR, to estimate the number of repeats in individuals. Show where in the region you would want PCR primers to hybridize, and on the gel below the diagram show the relative sizes of the PCR products that would be produced from a normal individual and from an affected individual. What size difference would you expect to see between the PCR products?
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This note was uploaded on 06/05/2009 for the course BIO 105 taught by Professor Sullivan during the Spring '08 term at University of California, Santa Cruz.

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HW key - 1 BIOL105ProblemSet#7 KEY Name DS TA Textbookproblems ,problems3,4,8,11,12,15a,b,16,17,18,19,21,26 ,problemsE5,E6,E7 Idon',

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