lecture_10_notes - Poisons • arsenate inhibits pyruvate...

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Lecture 10 Respiration Pyruvate oxidation (not decarboxylation); lecture notes 9 were corrected on Wed 22 am Fermentation See lecture 9 Alcoholic fermentation CH3COCOOH -> CO2 + CH3-COH NADH + H+ + CH3-COH -> NAD+ + CH3-CH2OH Lactic fermentation NADH + H+ + CH3COCOOH -> NAD+ + CH3CHOHCOOH Overview of energy in cell Cytoplasm: Glycolysis and fermentation Mitochondrion: Matrix: Pyruvate oxidation, Citric acid cycle Inner membrane: e- transport chain Regulation Respiration is regulated at multiple levels. An example: Citric acid (produced in first step of citric acid cycle) is the allosteric regulation of phospho-fructokinase, an enzyme at beginning of glycolysis Integration Metabolism: the chemical changes in living cells The pathways of respiration are integrated with many other metabolic processes. For example: The citric acid cycle (C6 to C4) both provides and uses backbone molecules for amino acids and bases Fatty acids break down proceeds via acetyl-CoA >> citric acid cycle
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Unformatted text preview: Poisons • arsenate inhibits pyruvate dehydrogenase, blocking pyruvate oxidation • cyanide inhibits a iron-containing protein in the e- transport chain, blocking e- transport - 1 -Photosynthesis Overview • Organisms need to find energy: they eat other organisms. This is not self-sustaining • Some organisms capture energy from solar radiation • Process is divided in to two steps: • Turn energy of light into ATP and reducing power (NADPH), byproduct is O2: light reac-tions, thylakoid of chloroplast • Use ATP and NADPH to convert CO2 and H2O into sugar (C fixation): dark reactions, stro-ma of chloroplast Light energy capture • Light -> Chlorophyll -> high energy e- from H2O (--> O2) • chlorophyll is inside thylakoid membrane • high energy electron outside thylakoid enters e- transport chain • two uses • pump H+, form gradient, power ATPase, make ATP • H+ is higher inside thylakoid vescicles • reduce NADP+- 2 -...
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