BME_552_Electro_Record_Tech_2009_v1

BME_552_Electro_Record_Tech_2009_v1 - BME 552 Neural...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
BME 552 – Neural Prosthetics Berger/Weiland Neural Electrophysiological Recording Techniques: Subcellular, Cellular, and Multi-Cellular Levels of Analysis key words : neural, electrophysiological, recording
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bioelectrical Properties of the CNS batteries : potential differences due to differences in the concentration of ions across cell membranes current : flow of ions from one volume of the CNS to another volume of the CNS (intracellular or extracellular) resistance : or conductance, limit on the number of ions per unit time that can flow through a pore in the membrane
Background image of page 2
Classes of Recordings of CNS Activity
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Types of Electrodes -- 1 metal wires : single cell; used for extracellular recording; usually require etching to reduce size (to 2-5 µ m) and change shape (to conical); stainless steel, platinum “rods” or “pins” multiple metal wires : multiple cells; extracellular recording of “ensemble”, population activity; usually metal wires because of the difficulty of etching 50-100 metal rods multiple metal contacts : micro-fabricated or micro-machined material; extracellular recording of “ensemble”, population activity; relatively “stiff” materials, i.e., silicon, ceramic
Background image of page 4
Types of Electrodes -- 2 glass pipette (1) : single cell; used for intracellular recording; (can be used for extracellular recording as well); requires “pulling” the pipette from both ends while heating the center; filled with electrolyte, e.g., 3M KCl; tip diameter must be 1 µ m or less glass pipette (2) : single cell; used for “whole-cell recording ”; same as above except that tip diameter is typically larger than used for intracellular recording, e.g., 2-3 µ m fluorescent dyes : use of dyes sensitive to infrared, ultra-violet, etc., light; either injected into cell or into bathing fluid; dyes can be conjugated to dyes sensitive to activity-related ions (Na+) or voltage
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Types of Electrodes -- 3 EEG (1) : “electroencephalography”; metal, conductive disks (paste underneath) used to record extracellular brain activity from surface of the scalp; approximate regional domain of recording is 3-5 cm EEG (2) : “ECoG” or electrocorticography”; metal, conductive disks used to record extracellular brain activity from surface of the dura; thus, signal not attenuated by skull and skin; approximate regional domain of recording is 0.5-1.0 cm
Background image of page 6
Intracellular Recording R s = R e
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Electrode Tip Impedance / Tip Junction Potentials Glass electrodes have a resistance and a potential associated with their tips. Partially related to Ag/AgCl wire used to interface electrolyte with the
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 49

BME_552_Electro_Record_Tech_2009_v1 - BME 552 Neural...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online