Lec21 - Lecture 21 Eukaryotic Gene Regulation 3...

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Lecture 21 Eukaryotic Gene Regulation 3 Transcription Repression & Combinatorial Gene Control (Lodish 6th ed. Chapter 7) (L.5th edition Chapter 11)
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Transcription repressors are modular Flexible hinge DNA (recognition of speciFc DNA sequences) Repression domain DNA-binding domain core promoter GTFs & Pol II CACGTG Repressor-specific DNA recognition sequence “repression” GTFs = general transcription factors Repressor
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A B C TBP x Transcription Repression Mechanisms “quenching” TBP TAFs TFIID TAFs Prevents activators from contacting general transcription factors Prevents assembly of other GTFs and Pol II into a complete pre- initiation complex (PIC).
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Recruitment of corepressors that modify histones HDAC E D Heterodimerization with dominant- negative inhibitory factor (e.g. Id ) repressor corepressor Deacetylated nucleosomes tightly packed over TATA box e.g.:HDAC = h istone d eac etylase F Recruitment of corepressors that contact the general transcription machinery TATA Inhibitor (e.g.: Id, an inhibitor of MyoD) x Activator dimer (e.g. MyoD) Binding site for activator TATA Binding site for repressor TBP CoR Corepressor inhibits pre-initiation complex (PIC) assembly (e.g.: NC2, negative cofactor 2) Binding site for repressor GTFs/Pol II TBP x Excess inhibitor
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Sin3-HDAC complex HDAC HDAC = Histone deacetylase Lodish 6th. Ed. Fig. 7-38
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SAGA complex HAT HAT = Histone acetyl-transferase Lodish 6th. Ed. Fig. 7-38
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This note was uploaded on 06/08/2009 for the course PHYS 2c taught by Professor All during the Spring '08 term at UC Riverside.

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Lec21 - Lecture 21 Eukaryotic Gene Regulation 3...

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