Introduction LR2

Introduction LR2 - Introduction We start this lab off using...

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Introduction We start this lab off using a process known as Polymerase Chain Reaction also called PCR. PCR enables the amplification of enormous amounts of DNA sequences and only requires small amounts DNA to begin with which does not need to be purified. Due to the high efficiency of PCR to amplify DNA sequencing, sensitivity to contamination becomes highly sensitive and so care must be taken not to introduce foreign DNA. We use this process to attempt to amplify the region containing the LuxAB genes from DNA purified from V. fischeri. Vibrio fischeri is a bacterium found in marine life environments. It has a unique ability to give off light, a phenomenon called Bioluminescence. The Lux operon is a set of genes found in the V. fischeri DNA which is responsible for the phenomenon bioluminescence. More specifically, we will be interested in the LuxAB gene found in the Lux operon, which produces the bioluminescence. After obtaining the LuxAB gene we will attempt to clone the PCR products into a vector
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This note was uploaded on 06/10/2009 for the course BICD 655698 taught by Professor Gustafson-brown,cindy during the Winter '09 term at UCSD.

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Introduction LR2 - Introduction We start this lab off using...

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