03 - MIC170 4/7/09 Lecture 3 (Tuesday, 4/7) Yeast cell 1....

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MIC170 – 4/7/09 1 Lecture 3 (Tuesday, 4/7) Yeast cell 1. Cell surface 2. Nucleus 3. Mitochondria 4. Vesicular organelles 5. Cytoskeleton 2. Nucleus (continued from the last lecture) Essential elements in yeast linear chromosomes 1) CEN 2) ARS 3) TEL 1) CEN (centromere) sequences ------ One per chromosome (handout KS-2) = Sites of kinetochore formation ----- microtubules attach to kinetochore to separate sister chromatids in mitosis Fig. 5.19 from Textbook -----------------> In budding yeast, 125-bp DNA is sufficient for the centromere function <- many different proteins bind to form kinetochore Comparison of centromere DNA structures ( Fig. 5.21 from Textbook)
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MIC170 – 4/7/09 2 2) ARS (Autonomously Replicating Sequence) ----- ~100bp First identified as sequences that enable plasmid DNA to replicate autonomously ( Fig. 6.14 from Textbook) – turned out it contains replication origin ORC (origin recognition complex) , which contains six different proteins, binds to origin to initiate DNA replication Budding yeast ARS (Fig. 6.15 from Textbook) ORC proteins turned out to be conserved also in humans.
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03 - MIC170 4/7/09 Lecture 3 (Tuesday, 4/7) Yeast cell 1....

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