05 - MIC170 4/14/09 Lecture 5 (Tuesday, 4/14) Yeast life...

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MIC170 – 4/14/09 1 Lecture 5 (Tuesday, 4/14) Yeast life cycle C. Mating, continued 2. Mating types are genetically determined by the mating type locus (on chromosome III) called “ MAT locus” MAT a a cell MAT α α cell Fig.2 (handout KS-4, p.2) MAT α encodes two proteins, α 1 and α 2, which are transcription factors α 1 activates transcription of α -specific genes ( α sg) ( α sg = α -factor and a -factor receptor genes) α 2 supresses transcription of a -specific genes ( a sg) ( a sg = a -factor and α -factor receptor genes) hsg = haploid-specific genes inhibit meiosis MAT a encodes a protein a 1 a 1 alone does not affect gene expression a / α diploid α 2 suppresses a -specific genes (asg) α 2 also forms a complex with a 1 suppresses expression of α 1 no α sg expression suppresses expression of hsg ready for meiosis
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MIC170 – 4/14/09 2 Homothallism = the property of being self-fertile In most of wild yeast strains, single haploid cell can give rise to diploid
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05 - MIC170 4/14/09 Lecture 5 (Tuesday, 4/14) Yeast life...

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